CAIK is a part of KFRI which is an institution of KSCSTE.
CAIK Analytical Services
Analytical Services
by Sophisticated Instrumental methods.
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We are located at KSCSTE-KFRI campus, Peechi. Thrissur-680653.

Course Code:CAIK-STC-02

Training programme on Gas Chromatography (ECD, FID & GCMS)

Gas Chromatography (GC) with Mass Spectrometry (MS) is one of the most important tools for quantitative identification of volatile and semi-volatile organic compounds in a complex mixture. Gas chromatography have many practical applications, such as, in petrochemical industries, natural products, cosmetics, pharmaceuticals, food, agriculture, herbal industries, forensics, environmental testing, pesticide analysis, explosives detection and other laboratory technology fields.

Our training program provides comprehensive training in Gas Chromatography with FID, ECD and MS Detectors under which full knowledge of detailed description of the instruments, its theory and the use of their accessories is imparted. Wide coverage is given to fundamental concepts of laboratory operations in a lucid manner so that both the novice and expert will find the programme as a useful reference for the day to day laboratory operations. The training is carried out according to the specified protocols and fundamental principles of Gas Chromatography.

Targeted Trainees

  1. Students in disciplines of Chemistry, Pharmacy, Food & Nutrition, Polymer Science, Environmental science, and Agricultural Sciences.
  2. Scientists and Research scholors new to Gas Chromatography.
  3. Practicing Chemists or scientists wishing to refresh their knowledge of GC
  4. Practicing Chemists with experience of running GC methods, but looking to develop new methods and to learn new troubleshooting tips.

Minimum Eligibility Required:

The trainee must possess a graduation degree in science field or must be pursuing the same. No matter what your level of expertise, we offer a course thatís perfect for you.

Selection Procedure

Since hands on training is planned, participants has to be restricted to a manageable number, hence 10 students per batch will be accommodated for the programme. Students may be selected based on an interview.

Course Duration & Course Fee

6 days, Rs.2500/-

Next Program(s)

Next year schedule will be announced on 15th November, 2018.

Registration Procedure & Last Date of Registration

Download the application form and duly filled application form to be sent to

    The Course Co-ordinator
    Center for analytical instrumentation Kerala (CAIK)
    Kerala Forest Research Institute,
    Peechi, Thrissur - 680653,
    Kerala, India
Last date: 20 days prior to the course commencement date..


Training Module

Day 1

Introduction to Gas chromatography. The GC system and its components. GC accessories - septa, liners, seals. Separation principles - Efficiencies, Retention Time, Retention Factor, Separation Factor, Resolution, Selectivity and HETP theory.

Day 2

Different types of columns, Parameters affecting GC column selection. Packed Column Injection Port and Capillary Column Injection Port. Carrier gas selection and properties. Phase Composition, Column Length, Film Thickness. Column flow. The effects of temperature. Explanation on sample Preparation techniques/ Extraction techniques with demonstration.

Day 3

The Ideal Detector, Detector Characteristics, Thermal Conductivity, Flame Ionization, Electron Capture, Mass Spectral Detector, GC-MS, Principles of Mass Spectroscopy, Ionisation techniques, Scan modes, PICI & NICI (positive & negative chemical Ionisation), Sample introduction (injection techniques).

Day 4

Introduction to integrators and Data acquisition software. Tuning and Calibration, Calibration report generation. Set up method for Full Scan and Single Ion Monitoring Operation, On-column injection, Split/Split less injection.Quantitative analysis methods and calculation (ESTD, ISTD, NORM ).

Day 5

Method development and validation as per ISO/IEC guidelines. Examples and Case Studies.

Day 6

Maintenance of GC & GC-MS and Troubleshooting covering the problems related to Gas supply, inlet, auto-sampler, column and detector, Problems with peaks.