HPLC is a form of column chromatography that pumps a sample mixture or analyte in a solvent at high pressure through a column with chromatographic packing material. The different components in the mixture pass through the column and the constituents of a sample can be identified by comparison of retention times with those of known standards run under the same conditions. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase. Today, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods.

Applications

• Separation of large molecular weight materials that have very low volatility Analysis of biologically important aromatic amines
• Determination of pharmaceutical products such as antibiotics, sedatives, steroids and analgesics
• Determination of artificial sweeteners, antioxidants, aflatoxins, amino acids, proteins, carbohydrates, lipids and vitamins

Specifications

System controller : SCL-10Avp
PDA detector : SPD-M10 Avp
Fluorescence detector : RF-20A
Solvent delivery pump: LC 10ATvp
Column oven : CTO-10ASvp
Software : LC Solutions

Instructions

• Samples must be extracted out in methanol or water
• If solvent free extract is being given for the analysis, please mention the solvent which was used for the extraction
• Submit samples in air tight glass vials
• For standards give minimum 2-5 mg, give preparation method and remaining standard can be collected back.

Sample Requirement

1g (in fine powder form) / 2ml (in liquid form)

Instrument Status

Working

Analytical Charges