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Reversed Phase - High Performance Liquid Chromatography (RP-HPLC)

Make: Shimadzu Corporation, Japan
Model: Capcellpak C18: 250x4.6mm

CAI-K Reversed Phase - High Performance Liquid Chromatography (RP-HPLC)

HPLC is a form of column chromatography that pumps a sample mixture or analyte in a solvent at high pressure through a column with chromatographic packing material. The different components in the mixture pass through the column and the constituents of a sample can be identified by comparison of retention times with those of known standards run under the same conditions. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase. Today, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods.

Applications

  • Separation of large molecular weight materials that have very low volatility Analysis of biologically important aromatic amines
  • Determination of pharmaceutical products such as antibiotics, sedatives, steroids and analgesics
  • Determination of artificial sweeteners, antioxidants, aflatoxins, amino acids, proteins, carbohydrates, lipids and vitamins

Specifications

System controller : SCL-10Avp
PDA detector : SPD-M10 Avp
Fluorescence detector : RF-20A
Solvent delivery pump: LC 10ATvp
Column oven : CTO-10ASvp
Software : LC Solutions

Instructions

  • Samples must be extracted out in methanol or water
  • If solvent free extract is being given for the analysis, please mention the solvent which was used for the extraction
  • Submit samples in air tight glass vials

Sample Requirement

1g (in fine powder form) / 2ml (in liquid form)

Instrument Status

Working

Analytical Charges

Facility/ Equipment Rates for sample analysis (Rs) Service tax -18 % extra)
KSCSTE Labs Edu. Insti. Govt R&D Labs Industries
HPLC 370.00 735.00 1470.00 2205.00
For quantitative analysis in HPLC, standards will be counted as individual samples.