CAIK is a part of KFRI which is an institution of KSCSTE.
CAIK Analytical Services
Analytical Services
by Sophisticated Instrumental methods.
CAIK Analytical Charges
Analytical Charges
Instrument wise charges and mode of payment..

Reversed Phase - High Performance Liquid Chromatography (RP-HPLC)

Make: Shimadzu Corporation, Japan
Model: Nexera Series

CAI-K Reversed Phase - High Performance Liquid Chromatography (RP-HPLC)

HPLC is a form of column chromatography that pumps a sample mixture or analyte in a solvent at high pressure through a column with chromatographic packing material. The different components in the mixture pass through the column and the constituents of a sample can be identified by comparison of retention times with those of known standards run under the same conditions. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase. Today, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods.


  • Separation of large molecular weight materials that have very low volatility Analysis of biologically important aromatic amines
  • Determination of pharmaceutical products such as antibiotics, sedatives, steroids and analgesics
  • Determination of artificial sweeteners, antioxidants, aflatoxins, amino acids, proteins, carbohydrates, lipids and vitamins


    System controller : SCL-10Avp
    PDA detector : SPD-M10 Avp
    Fluorescence detector : RF-20A
    Solvent delivery pump: LC 10ATvp
    Column oven : CTO-10ASvp
    Software : LC Solutions


  • Samples must be extracted out in methanol or water
  • If solvent free extract is being given for the analysis, please mention the solvent which was used for the extraction
  • Submit samples in air tight glass vials
  • For standards give minimum 2-5 mg, give preparation method and remaining standard can be collected back.

Sample Requirement

1g (in fine powder form) / 2ml (in liquid form)

Instrument Status


Analytical Charges

Facility/ Equipment Rates for sample analysis (Rs) Service tax -18 % extra)
KSCSTE Labs Edu. Insti. Govt R&D Labs Industries
HPLC 400.00 800.00 1600.00 2400.00
For quantitative analysis in HPLC, standards will be counted as individual samples.